Biological specimens are three-dimensional (3D), but most imaging technologies typically produce 2D pictures. Using chemical markers, such as fluorescent probes, combined with optical sectioning and superresolution techniques, scientists can now obtain high-resolution 3D renderings that provide a greater understanding of the structure–function relationship in cells and tissues. Likewise, advances in 3D scanning electron microscopy (SEM) approaches—including array tomography of serial sections, or serial block-face imaging with a focused ion beam (FIB) or an in situ microtome—are now carving a similar niche for nanoscale-resolution 3D imaging of biological specimens. Supported by a host of powerful hardware and software advancements, the versatile SEM instrument can now be used to generate near transmission electron microscopy (TEM)–resolution 3D ultrastructural images of stained, resin-embedded biological samples too large to be easily imaged by conventional EM methods. Improvements in the productivity and automation of EM instrumentation that allow the generation of high-resolution 3D images are producing considerable amounts of data, previously too difficult or time-consuming to collect. This webinar will focus on recent technical developments in 3D EM that enable imaging of a greater variety of biological samples, revealing unexpected and new biology.

During the webinar, the speakers will:

• Demonstrate how cells and tissue can be visualized in 3D by array tomography, scanning block-face SEM, and FIB-SEM imaging
• Discuss how researchers are increasing the scope and scale of 3D SEM imaging as well as its integration with other imaging modalities, including TEM and fluorescence microscopy
• Highlight new ways to connect light, electron, and X-ray microscopy platforms in a targeted, correlative, multiscale approach, emphasizing the use of ambient and cryo-light for electron microscopy workflows.

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